laminin coating coverslips

4/pack, 12/case. The dried coverslips can now be stored at room temperature until use. Overnight coating at +2°C to +8°C is recommended for a more reliable coating. The quality of adherent cells in culture depends on the substrate cells attached to because 50% cell surfaces are occupied by the substrate. ("IntersectionObserver"in window)&&i.e(39).then(i.t.bind(null,750,7)),! Poly-D-lysine (PDL) helps the link between laminin and glass and solve the problem. ._1tRa9{margin:0 10px;opacity:.6}.tnyql ._1728T{border:solid var(--brw,1px) rgba(var(--brd,var(--color_15)),var(--alpha-brd,1));box-shadow:var(--shd,0 1px 4px rgba(0,0,0,.6));border-radius:var(--rd,0)}.tnyql ._1lgpg{height:100%;width:100%;position:relative;border-bottom:solid var(--sepw,1px) rgba(var(--sep,var(--color_15)),var(--alpha-sep,1))}.tnyql ._1lgpg:last-child{border-bottom:0}.tnyql ._20FSe{box-sizing:border-box;position:absolute;min-width:100%;z-index:999;visibility:hidden;border:solid var(--brw,1px) rgba(var(--brd,var(--color_15)),var(--alpha-brd,1));border-radius:var(--SKINS_submenuBR,0);box-shadow:var(--shd,0 1px 4px rgba(0,0,0,.6))}.tnyql ._20FSe._1A4f5{left:calc(100% + var(--SKINS_submenuMargin, 8px))}.tnyql ._20FSe._2BZLn{right:calc(100% + var(--SKINS_submenuMargin, 8px))}.tnyql ._20FSe._9n91R{bottom:var(--brw,1px)}.tnyql ._20FSe._1i_hY{top:calc(-1*var(--brw, 1px))}.tnyql ._20FSe:before{content:" ";height:100%;width:var(--SKINS_submenuMargin,8px);position:absolute;top:0;right:var(--sub-menu-open-direction-right,auto);left:var(--sub-menu-open-direction-left,0);margin-right:calc(-1*var(--SKINS_submenuMargin, 8px));margin-left:calc(-1*var(--SKINS_submenuMargin, 8px))}.tnyql ._3gjfy{background-color:rgba(var(--bg,var(--color_11)),var(--alpha-bg,1));height:var(--item-height,50px);transition:var(--itemBGColorTrans,background-color .4s ease 0s)}.tnyql ._3gjfy.F5TY1>._3wg-V>._1FDdD{cursor:default}.tnyql ._3gjfy.SJr7i{background-color:rgba(var(--bgs,var(--color_15)),var(--alpha-bgs,1))}.tnyql ._3gjfy.SJr7i>._3wg-V>._1FDdD{color:rgb(var(--txts,var(--color_13)))}.tnyql ._3gjfy._1kReR>._20FSe{visibility:visible}.tnyql ._3gjfy._1kReR:not(.F5TY1){background-color:rgba(var(--bgh,var(--color_15)),var(--alpha-bgh,1))}.tnyql ._3gjfy._1kReR:not(.F5TY1)>._3wg-V>._1FDdD{color:rgb(var(--txth,var(--color_13)))}.tnyql ._20FSe ._3gjfy{background-color:rgba(var(--SKINS_bgSubmenu,var(--color_11)),var(--alpha-SKINS_bgSubmenu,1))}.tnyql ._20FSe ._1lgpg ._3gjfy{border-radius:0}.tnyql ._1lgpg:first-child ._3gjfy{border-radius:var(--rd,0);border-bottom-left-radius:0;border-bottom-right-radius:0}.tnyql ._1lgpg:last-child ._3gjfy{border-radius:var(--rd,0);border-top-left-radius:0;border-top-right-radius:0;border-bottom:0 solid transparent}.tnyql ._1FDdD{box-sizing:border-box;cursor:pointer;color:rgb(var(--txt,var(--color_15)));font:var(--fnt,var(--font_1));position:relative;display:flex;justify-content:var(--text-align,flex-start);height:100%;padding-left:var(--textSpacing,10px);padding-right:var(--textSpacing,10px);white-space:nowrap}.tnyql ._1FDdD,.tnyql ._1FDdD:after{background-color:transparent;width:100%}.tnyql ._1FDdD:after{cursor:default;content:" ";height:var(--sepw,1px);position:absolute;top:100%;left:0}.tnyql ._1lgpg ._3gjfy ._1FDdD{line-height:var(--item-height,50px)}.tnyql ._20FSe ._1FDdD{font:var(--SKINS_fntSubmenu,var(--font_1))} HCl-washed and aminopropyltriethoxysilane (APTES, Acros Organics) treated coverslips were incubated for 3 hours at 37°C with 1–2 mg/mL laminin … 1. All proteins were added in excess to ensure equal coating. (!e||"object"!==r(e))&&Object.keys(e).some((function(i){return e[i]===t}))},last:function(e){return e[e.length-1]},template:function(e){return function(t){var i=e;for(var r in t)t.hasOwnProperty(r)&&(i=i.replace(new RegExp("\\${"+r+"}","g"),t[r]));return i}}}},function(e,t,i){"use strict";var r={isWEBP:{lossless:!1,lossy:!1,alpha:!1,animation:!1},isObjectFitBrowser:!0};e.exports={getFeature:function(e){return r[e]},setFeature:function(e,t){r[e]=t}}},function(e,t,i){"use strict";function r(e,t){var i=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter((function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable}))),i.push.apply(i,r)}return i}function n(e,t,i){return t in e?Object.defineProperty(e,t,{value:i,enumerable:!0,configurable:!0,writable:!0}):e[t]=i,e}var a=i(1),o=i(7),s=i(8),c=i(0);e.exports={getTransform:function(e,t,i,r){var n=a.isSEOBot(r),c=a.getFileType(t.id),u=a.getFileName(t.id,t.name),l=a.getFileExtension(t.id),h=!n&&a.isWEBPBrowserSupport(c),d=n?1:a.getDevicePixelRatio(i),f={fileName:u,fileExtension:l,fileType:c,isWEBPSupport:h,fittingType:e,preferredExtension:h? Fibronectin was prepared from human plasma (18). Coating of Cell Culture Plate Note: Preparation of the cell culture plates should be done in a laminar flow cell culture hood. These coverslips… We further analyzed the signaling factors that might be involved in this process of differentiation using real-time PCR and qPCR analysis after 7 days of culture. Without a laminin coating, neurons could hardly survive the uncoated surfaces ( Fig. ]+)/),l=null===u.exec(e)?null:parseFloat(u.exec(e)[1]),h=function(e,t,i,r,n){var a=!e.phone&&!e.tablet&&i.chrome&&r>=23,o=e.android&&(e.phone||e.tablet)&&i.webkit&&i.chrome&&r>=25,s=e.android&&n<535&&(e.phone||e.tablet)&&t>=4.2&&i.webkit&&!i.safari,c=i.edge&&r>=18,u=!e.firefoxos&&i.firefox&&!i.webkit&&r>=65;return!! • The laminin matrix can easily be used for coating of glass. Corning™ BioiCoat™ 12 mm No. A chambered coverslip with 8 wells for cell culture, immunofluorescence, and high-end microscopy. 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